Composite

Part:BBa_K4760007:Design

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-10)


Lpp-OmpA-SpyTag003


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 463
    Illegal BamHI site found at 475
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This surface display strategy is the only one we had enough time to test. The proteins might be not outstreched enough, about which we welcome further testing and improvement.


Source

Both parts used are optimized reported version for better efficiency. In our project, they're synthesized and assemblied.

References